Journal: Bioactive Materials

Article Title: Microenvironment-responsive injectable dynamic hydrogel for sequential antioxidant and tissue regeneration therapy of radiation-induced skin injury ☆

doi: 10.1016/j.bioactmat.2026.03.057

Figure Lengend Snippet: The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

Article Snippet: The Live/Dead Cell Double Staining Kit was supplied by Jiangsu KeyGEN BioTECH Co., Ltd., and the Cell Counting Kit-8 (CCK-8) was obtained from Beijing Solarbio Science & Technology Co., Ltd.

Techniques: Activity Assay, Incubation, Staining, Co-Culture Assay

Journal: Materials Today Bio

Article Title: 3D-printed titanium scaffolds coated with a multifunctional photothermal-responsive hydrogel promote osteoporotic bone defect repair

doi: 10.1016/j.mtbio.2026.102879

Figure Lengend Snippet: Biocompatibility and cell adhesion of the bone marrow mesenchymal stem cells (BMSCs) in different groups. (A) Cell viability at day 1, 4 and 7 via the CCK-8 assay. (B) Percentage of live cells at day 1 assessed by Live/Dead staining. (C) Proportion of adherent BMSCs. (D) Representative fluorescence images of BMSCs obtained by Live/Dead staining. (E) Representative fluorescence images of adherent BMSCs stained with phalloidin. ∗∗ p < 0.01, and ∗∗∗ p < 0.001, n = 3.

Article Snippet: A live/dead cell double-staining kit (KGA9501-100) was obtained from KeyGENBiotech (Nanjing, China), and a CCK-8 assay kit (TM772) was purchased from Dojindo (Tokyo, Japan).

Techniques: CCK-8 Assay, Staining, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Mechanism of Yiqi Huoxue Granule in promoting angiogenesis of skin lesion tissue by increasing CSF2 to reduce mesenchymal stem cell apoptosis

doi: 10.3389/fphar.2026.1649017

Figure Lengend Snippet: Yiqi Huoxue Granule alleviates hypoxia - induced MSC apoptosis and promotes tube formation. Note: (A1–5) Flow cytometry diagrams; (A6) CCK8 result analysis diagram, n = 3; (A7) Flow cytometry result analysis diagram, n = 3; (B1–5) Three - dimensional tube formation diagrams; (B6) Tube formation result analysis diagram, n = 5; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After 24 h of hypoxia intervention, use the Annexin V - FITC/PI double - staining cell apoptosis detection kit (KeyGen Biotech, China, lot: 20211224) for detection.

Techniques: Flow Cytometry

Journal: Frontiers in Pharmacology

Article Title: Mechanism of Yiqi Huoxue Granule in promoting angiogenesis of skin lesion tissue by increasing CSF2 to reduce mesenchymal stem cell apoptosis

doi: 10.3389/fphar.2026.1649017

Figure Lengend Snippet: Transcriptomic results of Yiqi Huoxue Granule in the intervention of hypoxic MSC. Note: (A) Bubble chart of apoptosis - related biological processes; (B) Screening of apoptosis - related genes; (C) Volcano plot of apoptosis - related differential genes; (D) Bar chart of apoptosis - related differential genes.

Article Snippet: After 24 h of hypoxia intervention, use the Annexin V - FITC/PI double - staining cell apoptosis detection kit (KeyGen Biotech, China, lot: 20211224) for detection.

Techniques: